1. The restriction enzymes consist of the following enzymatic elements
a) Nuclease and protease b) Nuclease and kinase c) Nuclease and methylase d) Nuclease and polymerase
2. A mRNA that includes coding regions of more than one gene
a) Polysomes b) Polycistronic c) Poly linker d) Polyadenylated
3. RNA molecule that can catalyze chemical reactions, often cutting other RNAs
a) Ribonuclease b) Ribozyme c) R- Zyme d) Ribofactor
4. A gene from one genome that has been incorporated into the genome of another organism
a) Tra gene b) Cis gene c) Mob gene d) Transgene
5. Eukaryotic promoter region analogous to the pribnow box
a) Shine- Delgarno sequence b) Pribnow box c) TATA box d) CAAT box
6. The bar gene generally used as a selectable marker gene during the transgenic development imparts
a) Antibiotic resistance b) Drug resistance c) Herbicide tolerance d) High Amino acid tolerance
7. The deletion of genes governing auxin and cytokinin production from T- DNA of a Ti plasmid is known as
a) Disharming b) Disarming c) Dearming d) Deharming
8. An individual with identical alleles are known as
a) Heterozygous b) Homozygous c) Homogeneous d) Heterogenous
9. The individual with AaBbCcDD can produce the following number of gametes
a) Eight b) Nine c) Sixty four d) Twenty-seven
10. Under physiological conditions the dominant form of DNA
a) B- form b) A- Form c) Z- form d) D form
11. The part of the protein coding gene that is translated into protein is
a) Codon b) Exon c) Open reading frame d) Closed reading frame
12. The process of separation of two strands of the DNA by subjecting to high temperature is
a) Denaturation b) Renaturation c) Deformation d) Conformation
13. Amount of DNA in the haploid genome of an eukaryotic cell is known as
a) Tm value b) C- value c) D -Value d) Cot value
14. The process of transfer of biological information from RNA to DNA in certain viruses was discovered by
a) Francis Crick, 1958 b) Baltimore ,1970 c) Hargobind Singh Khorana, 1970 d) Watson , 1970
15. Operons are a common feature in the organization of genomes in
a) Bacteria b) Fungi c) Animals d) Plants
16. In eukaryotes the synthesis of tRNA is catalyzed by
a) RNA polymerase II b) RNA polymerase I c) RNA polymerase III d) RNA polymerase IV
17. A frame shift mutation can be a result of
a) Transversion b) Transiition c) Base substitution d) A Single base pair addition
18. Restricted Fragment Length Polymorphism markers are
a) Dominant b) Co-dominant c) Incomplete dominant d) Non dominant
19. Hershey and Chase demonstrated that DNA as the genetic material with following
a) E. coli and M13 phage b) E. coli and T2 phage c) E. coli and P1 phage d) E. coli and P2 phage
20. GEAC stands for
a) Genetic Engineering Appraisal Committee b) Genetic Engineering Committee for Agriculture c) Genetic Engineers Association at Central level d) Genetic Engineering Acceptance Committee
21. DNA fragments that differ in lengths by just one nucleotide can be separated by
a) Agarose gel electrophoresis b) Polyacrylamide gel electrophoresis c) Denaturing gel electrophoresis d) Gel filtration
22. Normally self ligation of the vector DNA molecules are prevented
a) By adding phosphate group to the cut ends of the vector b) By removing the phosphate group from the cut ends of the vector c) By methylation at cut ends d) By demethylation at cut ends
23. Single stranded DNA suitable for sequencing can be obtained by
a) Plasmid based vectors b) Cosmid based vectors c) M13 based vectors d) Shuttle vectors
24. Restriction endonucleases cannot restrict host DNA because
a) Methylation of nucleotides in the genome b) Methylation of nucleotides at the recognition sites c) Demethylation of the of nucleotides in the genome d) Demethylation of the nucleotides at the recognition sites
25. In recombinant DNA technology for gene cloning purpose, it is desirable to use the following DNA polymerase
a) DNA polymerase I b) Taq DNA polymerase c) Pfu polymerase d) DNA polymerase III
26. If the GC content in the DNA is higher, the Tm value is
a) Higher b) Lower c) GC content has no influence on Tm value d) GC content has least influence on Tm value
27. A genetic test used to determine the number of genes responsible for a phenotype
a) Blue –white colony assay b) Complementation test c) Two point test cross d) Yeast two hybrid assay
28. The following scientists provided the first evidence for conjugation
a) Beadle and Tatum b) Lederberg and Tatum c) Frankel Conrat and Singer d) Benzer and Crick
29. A discrete DNA entity which moves between DNA sites that lack homology using self encoded enzyme is
a) Transposons b) T-DNA c) Vector d) Bacteriophages
30. Ribosome is a site of
a) Protein synthesis b) Fatty acid synthesis c) Carbohydrate synthesis d) rRNA synthesis
31. Enzyme involved in removal of primers and filling up of gaps in lagging strands is
a) Ligase b) Helicase c) DNA polymerase III d) DNA polymerase I
32. The in vitro synthesis of DNA was first accomplished by
a) Arthur Kornberg b) Kary Mullis c) Meselson and Stahl d) Frankel Conrat and Sanger
33. Radioactive isotopes 32P and 35S were used in the experiments which demonstrated
a) DNA is the genetic material b) Semiconservative replication of DNA c) Discontinuous DNA synthesis in one strand d) Protein synthesis in E. coli
34. An organelle present in animal cells but missing from plant cells is
a) A nucleolus b) A centriole c) Vacuole d) Mitochandia
35. Chemical used to double the chromosome number
a) Acetocarmine b) Acetoorcein c) Florescein diacetate d) Colchicine
Answers
1.C 10.A 20.A 30.A
2.B 11.B 21.B 31.A
3.B 12.A 22.B 32.D
4.D 13.B 23.C 33.A
5.C 14.B 24.B 34.B
6.C 15.A 25.C 35.D
7.B 16.C 26.A
8.B 17.D 27.B
9.A 18.B 28.B
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